Exocytosis, part of which is the merger of the vesicle and the plasma membrane, is charac­teristic for eukaryotic cells, which engage this process in a myriad of cell functions. For a long time it was believed that exocytosis was predominantly regulated by proteins, a view refer­red to as ‘proteocentric’. Only recently it was shown that many of lipids, previously conside­red as passive cell membrane components, also play vital roles in regulation of exocytosis. Among others, such lipids are phosphatidylinositol-4,5-biphosphate, diacylglycerol, polyun­saturated fatty acids, sphingosine and cholesterol. Much like proteins, lipids act at various points in the process and influence exocytosis in various ways. The goal of this article is to high­light the role of lipids in exocytosis.