Lateral redistribution of molecules in cell membrane may result in budding and shedding of vesicles smaller than micrometer, called microvesicles. Microvesicles reflect in their cha­racteristics the state and type of cells wherefrom they originate. The state of cells may be changed in diseases therefore also microvesicles, which originate from those cells, characte­ristically represent the disease and are an interesting potential diagnostic tool. Despite nume­rous studies and publications there is still no standard protocol for microvesicle isolation and characterisation from the peripheral blood. In this work we used electron microscopy as a pos­sible tool for characterisation of microvesicles and for determination of possible artefacts acqui­red in the isolation procedure. Scanning electron microscopy proved useful for determination of three-dimensional form of microvesicles. Transmission electron microscopy enabled the insight into inner structure of microvesicles.