{"id":1784,"date":"2013-08-19T15:35:55","date_gmt":"2013-08-19T15:35:55","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=1784"},"modified":"2013-08-19T15:35:55","modified_gmt":"2013-08-19T15:35:55","slug":"disease-proteome-analysis-with-two-dimensional-gel-electrophoresis-and-mass-spectrometry","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/disease-proteome-analysis-with-two-dimensional-gel-electrophoresis-and-mass-spectrometry\/","title":{"rendered":"Disease Proteome Analysis with Two-Dimensional Gel Electrophoresis and Mass Spectrometry"},"content":{"rendered":"<div>\n<p>A two-dimensional gel electrophoresis is one of the basic techniques of proteomics. It is used to separate proteins. As already hinted by the name, we are using the method to separate pro\u00adteins in two dimensions. Firstly, they are separated during isoelectric focusing according to their isoelectric points and then according to their molecular weight during sodium dodecyl sulphate polyacrylamide gel electrophoresis &#8211; resulting in a gel with round spots of separated proteins. The method is usually used in combination with mass spectrometry and bioinformatics: the first one enables us to identify the desired proteins (i. e. those with differential expression in compared samples), and the second one enables us to use different computer programes for determining the spots (proteins) with differential expression, as well as identifying them by using different databases. In this article we methodologically describe phases of proteome investigation, from sample preparation and two-dimensional gel electrophoresis to gel analysis with mass spectrometry and protein identification. We are showing the possibilities of using the method in biomedicine, with particular focus on our research of oncogenesis at gastric mucosa.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A two-dimensional gel electrophoresis is one of the basic techniques of proteomics. It is used to separate proteins. As already hinted by the name, we are using the method to separate pro\u00adteins in two dimensions. Firstly, they are separated during isoelectric focusing according to their isoelectric points and then according to their molecular weight during [&hellip;]<\/p>\n","protected":false},"author":30,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[380,412],"tags":[],"class_list":["post-1784","post","type-post","status-publish","format-standard","hentry","category-2-2008-archive","category-biochemistry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Disease Proteome Analysis with Two-Dimensional Gel Electrophoresis and Mass Spectrometry - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Disease Proteome Analysis with Two-Dimensional Gel Electrophoresis and Mass Spectrometry - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"A two-dimensional gel electrophoresis is one of the basic techniques of proteomics. It is used to separate proteins. As already hinted by the name, we are using the method to separate pro\u00adteins in two dimensions. Firstly, they are separated during isoelectric focusing according to their isoelectric points and then according to their molecular weight during [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medrazgl.si\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\/\" \/>\n<meta property=\"og:site_name\" content=\"Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"article:published_time\" content=\"2013-08-19T15:35:55+00:00\" \/>\n<meta name=\"author\" content=\"\u010crt Zavrnik\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"\u010crt Zavrnik\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/\"},\"author\":{\"name\":\"\u010crt Zavrnik\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/80418a5a6b59976483b5d19fc87a9092\"},\"headline\":\"Disease Proteome Analysis with Two-Dimensional Gel Electrophoresis and Mass Spectrometry\",\"datePublished\":\"2013-08-19T15:35:55+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/\"},\"wordCount\":190,\"commentCount\":0,\"articleSection\":[\"2\",\"Biochemistry\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/preiskava-bolezenskih-proteomov-z-dvodimenzionalno-gelsko-elektroforezo-in-masno-spektrometrijo\\\/\",\"name\":\"Disease Proteome Analysis with Two-Dimensional Gel Electrophoresis and Mass Spectrometry - 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