{"id":2497,"date":"2013-08-26T16:55:14","date_gmt":"2013-08-26T16:55:14","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=2497"},"modified":"2013-08-26T16:55:14","modified_gmt":"2013-08-26T16:55:14","slug":"the-cytoprotective-effect-of-verapamil-on-the-ischemically-and-hypoxically-injured-isolated-rat-heart","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/the-cytoprotective-effect-of-verapamil-on-the-ischemically-and-hypoxically-injured-isolated-rat-heart\/","title":{"rendered":"The cytoprotective effect of verapamil on the ischemically and hypoxically injured isolated rat heart"},"content":{"rendered":"<p>Isolated perfused Langendorff rat \u2028hearts were used for comparing effects of the\u2028 calcium antagonist verapamil on the following \u2028two models of heart injury: local ischemia (ligature of the anterior interventricular artery) and\u2028 general hypoxia (perfusate saturated with nitrogen). The degree and extent of injury were\u2028 assessed by measuring the activity of lactate \u2028dehydrogenase in venous effluents from the \u2028heart, contractility, heart rate, coronary flow,\u2028and ECG. Lactate dehydrogenase activity in the\u2028 venous effluents was the marker of cellular \u2028injury. Verapamil had a beneficial effect on the \u2028contractility and rhythmicity after reoxygenation \u2028of the hypoxically injured heart. Verapamil significantly reduced the release of lactate dehydrogenase during reperfusion and reoxygenation of ischemic and hypoxic hearts, respectively. Maximal lactate dehydrogenase activities \u2028during reperfusion of the ischemic hearts, treated with verapamil, were 6.3 \u00b11.9 kat\/g x \u2028ml\/min, compared to activities obtained from\u2028 untreated ischemic hearts, 23.6 \u00b1 5.4 kat\/g x\u2028 ml\/min. Maximal lactate dehydrogenase activities during reoxygenation of the hypoxic hearts, treated with verapamil, were 3.6 \u00b1 0.9 kat\/g x\u2028ml\/min, compared to the activities of untreated \u2028hypoxic hearts, 8.0 \u00b1 2.4 kat\/g x ml\/min. Results of our experiment showed cytoprotective \u2028effects of verapamil, especially on local ischemic injury of the isolated heart.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Isolated perfused Langendorff rat \u2028hearts were used for comparing effects of the\u2028 calcium antagonist verapamil on the following \u2028two models of heart injury: local ischemia (ligature of the anterior interventricular artery) and\u2028 general hypoxia (perfusate saturated with nitrogen). The degree and extent of injury were\u2028 assessed by measuring the activity of lactate \u2028dehydrogenase in venous [&hellip;]<\/p>\n","protected":false},"author":25,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[303,418,408],"tags":[],"class_list":["post-2497","post","type-post","status-publish","format-standard","hentry","category-4-1992-archive","category-pharmacology-and-experimental-toxicology","category-preclinical-research-paper"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>The cytoprotective effect of verapamil on the ischemically and hypoxically injured isolated rat heart - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/citoprotektivni-ucinek-verapamila-pri-ishemicno-in-hipoksicno-okvarjenem-izoliranem-podganjem-srcu\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The cytoprotective effect of verapamil on the ischemically and hypoxically injured isolated rat heart - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Isolated perfused Langendorff rat \u2028hearts were used for comparing effects of the\u2028 calcium antagonist verapamil on the following \u2028two models of heart injury: local ischemia (ligature of the anterior interventricular artery) and\u2028 general hypoxia (perfusate saturated with nitrogen). 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