{"id":2684,"date":"2013-08-27T07:53:48","date_gmt":"2013-08-27T07:53:48","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=2684"},"modified":"2013-08-27T07:54:47","modified_gmt":"2013-08-27T07:54:47","slug":"application-of-polymerase-chain-reaction-in-gonorrhea-diagnosis","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/application-of-polymerase-chain-reaction-in-gonorrhea-diagnosis\/","title":{"rendered":"Application of Polymerase Chain Reaction in Gonorrhea Diagnosis"},"content":{"rendered":"<div>\n<p align=\"left\">Neisseria gonorrhoeae is a Gram negative diplococcus the cause of sexually transmitted dis\u00adease gonorrhea. It is transmitted through a promiscuous intercourse, from infected mother to the newborn and from one partner to another. The standard method of diagnostics of gon\u00adorrhea is the cultivation and identification of the bacteria, which is based on selective fermentation of different sugars. As the serological diagnostics is not sufficiently reliable, the polymerase chain reaction technique carries important advantages.<\/p>\n<\/div>\n<p>To asses the use of the polymearase chain reaction COBAS AMPLICOR as a diagnostic method for Neisseria gonorrhoeae infection and its reliability in comparison with the classi\u00adcal method.<\/p>\n<p align=\"left\">We divided the collected samples in three groups. Group A group of 113 clinical speci\u00admen (urine, cervical smear) from the Outpatient Clinic for Sexually Transmited Diseases, Department of Dermatology in Ljubljana, accounted as a population with high risk of infec\u00adtion; group B of 48 medical students (urine rinse of urethra), presumed to be a population with low risk of infection; group C of 31 frozen samples of six different strains from the genus Neisseriaceae that have shown the specificity of the COBAS AMPLICOR polymerase chain reaction test.<\/p>\n<p align=\"left\">In the group A we found three gonorrhea positive patients with the polymerase chain reaction method, as well as with the standard method of cultures and fermentation of sug\u00adars. All the results of the testing in the group B were negative. In the group C we correctly identified the 10 N. gonorrhoeae among the different Neisseriaceae strains.<\/p>\n<p>With our work we proved that COBAS AMPLICOR polymerase chain reaction test is use\u00adful for diagnostic and screening purposes. It is as reliable as classical diagnostic method and enough specific to discern N. gonorrhoeae from other bacteria of the genus of Neisseriaceae.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Neisseria gonorrhoeae is a Gram negative diplococcus the cause of sexually transmitted dis\u00adease gonorrhea. It is transmitted through a promiscuous intercourse, from infected mother to the newborn and from one partner to another. The standard method of diagnostics of gon\u00adorrhea is the cultivation and identification of the bacteria, which is based on selective fermentation of [&hellip;]<\/p>\n","protected":false},"author":23,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[343,432,408],"tags":[],"class_list":["post-2684","post","type-post","status-publish","format-standard","hentry","category-4-2000-archive","category-microbiology-and-immunology","category-preclinical-research-paper"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Application of Polymerase Chain Reaction in Gonorrhea Diagnosis - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Application of Polymerase Chain Reaction in Gonorrhea Diagnosis - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Neisseria gonorrhoeae is a Gram negative diplococcus the cause of sexually transmitted dis\u00adease gonorrhea. It is transmitted through a promiscuous intercourse, from infected mother to the newborn and from one partner to another. The standard method of diagnostics of gon\u00adorrhea is the cultivation and identification of the bacteria, which is based on selective fermentation of [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medrazgl.si\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\/\" \/>\n<meta property=\"og:site_name\" content=\"Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"article:published_time\" content=\"2013-08-27T07:53:48+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2013-08-27T07:54:47+00:00\" \/>\n<meta name=\"author\" content=\"O\u017ebej Kun\u0161i\u010d\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"O\u017ebej Kun\u0161i\u010d\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/\"},\"author\":{\"name\":\"O\u017ebej Kun\u0161i\u010d\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/afeabc2cf79a5c40cc7ed495b7961a9a\"},\"headline\":\"Application of Polymerase Chain Reaction in Gonorrhea Diagnosis\",\"datePublished\":\"2013-08-27T07:53:48+00:00\",\"dateModified\":\"2013-08-27T07:54:47+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/\"},\"wordCount\":298,\"commentCount\":0,\"articleSection\":[\"4\",\"Microbiology and Immunology\",\"Preclinical research paper\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/uporabnost-verizne-reakcije-s-polimerazo-v-diagnostiki-gonoreje\\\/\",\"name\":\"Application of Polymerase Chain Reaction in Gonorrhea Diagnosis - 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