{"id":3899,"date":"2013-09-07T13:30:06","date_gmt":"2013-09-07T13:30:06","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=3899"},"modified":"2013-09-07T13:30:06","modified_gmt":"2013-09-07T13:30:06","slug":"relationship-between-cytochrome-p450-cyp1a1-gene-polymorphism-and-lung-cancer-susceptibility-in-slovene-population","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/relationship-between-cytochrome-p450-cyp1a1-gene-polymorphism-and-lung-cancer-susceptibility-in-slovene-population\/","title":{"rendered":"Relationship between cytochrome P450 (CYP1A1) gene polymorphism and lung cancer susceptibility in Slovene population"},"content":{"rendered":"<p>Two polymorphic sites of the CYP1A1 gene, coding for cytochrome P4501A1 enzyme, have been discovered so far. One involves the 7<sup>th<\/sup> exon of the coding region; a nucleotide chan\u00adge leads to amino acid replacement in the heme binding region of cytochrome P4501A1 (poly\u00admorphism Ile\/Val). Another site is in the 3&#8242;-flanking region of the gene, and results in the presence of a new restriction site for the <em>Msp<\/em> I enzyme (polymorphism <em>Msp<\/em> I ). This study inve\u00adstigated the role of both polymorphisms in the development of lung cancer caused by chemi\u00adcal carcinogenesis. We analysed 156 samples obtained from patients with lung cancer, and 102 samples from healthy individuals. The met\u00adhod I used was based on amplification of the polymorphic part of the gene by polymerase chain reaction. To detect the polymorphism <em>Msp<\/em> I the products of reaction were analysed using the restriction and electrophoresis on agarose gel, while the presence of polymorphism Ile\/Val was determined using specific primers. For the identification of the reaction product elec\u00adtrophoresis on agarose gel was employed. A sta\u00adtistically significant association between polymorphism <em>Msp<\/em> I and polymorphism Ile\/Val was found in both lung cancer patients and healthy controls. There was no statistically sig\u00adnificant difference in the incidence of both poly\u00admorphism forms between lung cancer patients and controls or between patients with different histological subtypes of lung cancer.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Two polymorphic sites of the CYP1A1 gene, coding for cytochrome P4501A1 enzyme, have been discovered so far. One involves the 7th exon of the coding region; a nucleotide chan\u00adge leads to amino acid replacement in the heme binding region of cytochrome P4501A1 (poly\u00admorphism Ile\/Val). Another site is in the 3&#8242;-flanking region of the gene, and [&hellip;]<\/p>\n","protected":false},"author":18,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[330,412,424,408],"tags":[],"class_list":["post-3899","post","type-post","status-publish","format-standard","hentry","category-1-1998-archive","category-biochemistry","category-human-genetics","category-preclinical-research-paper"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Relationship between cytochrome P450 (CYP1A1) gene polymorphism and lung cancer susceptibility in Slovene population - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Relationship between cytochrome P450 (CYP1A1) gene polymorphism and lung cancer susceptibility in Slovene population - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Two polymorphic sites of the CYP1A1 gene, coding for cytochrome P4501A1 enzyme, have been discovered so far. One involves the 7th exon of the coding region; a nucleotide chan\u00adge leads to amino acid replacement in the heme binding region of cytochrome P4501A1 (poly\u00admorphism Ile\/Val). Another site is in the 3&#8242;-flanking region of the gene, and [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medrazgl.si\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\/\" \/>\n<meta property=\"og:site_name\" content=\"Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"article:published_time\" content=\"2013-09-07T13:30:06+00:00\" \/>\n<meta name=\"author\" content=\"Andra\u017e Nendl\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Andra\u017e Nendl\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/\"},\"author\":{\"name\":\"Andra\u017e Nendl\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/0e6cbf535aef1eb72431e6561d089434\"},\"headline\":\"Relationship between cytochrome P450 (CYP1A1) gene polymorphism and lung cancer susceptibility in Slovene population\",\"datePublished\":\"2013-09-07T13:30:06+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/\"},\"wordCount\":247,\"commentCount\":0,\"articleSection\":[\"1\",\"Biochemistry\",\"Human Genetics\",\"Preclinical research paper\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/povezava-polimorfizma-gena-za-citokrom-p450-cyp1a1-z-dovzetnostjo-za-pljucnega-raka-pri-slovencih\\\/\",\"name\":\"Relationship between cytochrome P450 (CYP1A1) gene polymorphism and lung cancer susceptibility in Slovene population - 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