{"id":4102,"date":"2013-09-08T09:41:24","date_gmt":"2013-09-08T09:41:24","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=4102"},"modified":"2013-09-08T09:41:24","modified_gmt":"2013-09-08T09:41:24","slug":"the-effect-of-neurotrophin-nt-3-on-functional-capabilities-of-motoneurons-in-cocultures-with-human-muscle","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/the-effect-of-neurotrophin-nt-3-on-functional-capabilities-of-motoneurons-in-cocultures-with-human-muscle\/","title":{"rendered":"The Effect of Neurotrophin NT-3 on Functional Capabilities of Motoneurons in Cocultures with Human Muscle"},"content":{"rendered":"<p>Neurotrophins are a subgroup of growth factors acting within the nervous system. They are small proteins forming a family of several members. Among these, NT-3 is the most abun\u00addantly distributed within the developing and mature nervous system. NT-3 significantly contributes to the formation and maintenance of the nervous system and may be involved in various neurological diseases. In the future, it may also become a therapeutic agent for their treatment, therefore the mechanisms of its action are intensely investigated. The aim of our study was to investigate the effects of NT-3 on the ability of motor neurons to form\u00a0functional neuromuscular junctions. An in vitro experimental model in which neurons extend\u00ading from rat spinal cord explants form functional neuromuscular junctions onto human myotubes has been employed for this purpose. The effects of NT-3 were quantitatively deter\u00admined by 1) counting neuronal outgrowths from the spinal cord explants 2) determining the percentage of contraction-positive explants 3) counting the number of contracting units per explant and 4) counting the number of nicotinic receptor clusters at neuromuscular junc\u00adtions using rhodamine-labelled a-bungarotoxin. The statistical significance of differences between the treated and control groups of explants was tested using the independent sam\u00adples Student t-test. NT-3 added to the culture medium at the concentration of 10 ng\/ml, which proved the most efficient in our concentration testing, increased all the parameters of func\u00adtional innervation. A higher capacity of the neurons to form functional neuromuscular junctions in NT-3-containing cocultures was thus demonstrated.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Neurotrophins are a subgroup of growth factors acting within the nervous system. They are small proteins forming a family of several members. Among these, NT-3 is the most abun\u00addantly distributed within the developing and mature nervous system. NT-3 significantly contributes to the formation and maintenance of the nervous system and may be involved in various [&hellip;]<\/p>\n","protected":false},"author":28,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[372,439,408],"tags":[],"class_list":["post-4102","post","type-post","status-publish","format-standard","hentry","category-4-2006-archive","category-pathophysiology","category-preclinical-research-paper"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>The Effect of Neurotrophin NT-3 on Functional Capabilities of Motoneurons in Cocultures with Human Muscle - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/vpliv-nevrotrofina-nt-3-na-funkcionalne-sposobnosti-motonevronov-v-kokulturi-s-clovesko-misico\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The Effect of Neurotrophin NT-3 on Functional Capabilities of Motoneurons in Cocultures with Human Muscle - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Neurotrophins are a subgroup of growth factors acting within the nervous system. They are small proteins forming a family of several members. Among these, NT-3 is the most abun\u00addantly distributed within the developing and mature nervous system. 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