{"id":4125,"date":"2013-09-08T12:06:01","date_gmt":"2013-09-08T12:06:01","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=4125"},"modified":"2013-09-09T10:23:05","modified_gmt":"2013-09-09T10:23:05","slug":"microvesicles-from-blood-plasma-observed-by-electron-microscopy","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/microvesicles-from-blood-plasma-observed-by-electron-microscopy\/","title":{"rendered":"Microvesicles from Blood Plasma Observed by Electron Microscopy"},"content":{"rendered":"<div>\n<p align=\"left\">Lateral redistribution of molecules in cell membrane may result in budding and shedding of vesicles smaller than micrometer, called microvesicles. Microvesicles reflect in their cha\u00adracteristics the state and type of cells wherefrom they originate. The state of cells may be changed in diseases therefore also microvesicles, which originate from those cells, characte\u00adristically represent the disease and are an interesting potential diagnostic tool. Despite nume\u00adrous studies and publications there is still no standard protocol for microvesicle isolation and characterisation from the peripheral blood. In this work we used electron microscopy as a pos\u00adsible tool for characterisation of microvesicles and for determination of possible artefacts acqui\u00adred in the isolation procedure. Scanning electron microscopy proved useful for determination of three-dimensional form of microvesicles. Transmission electron microscopy enabled the insight into inner structure of microvesicles.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Lateral redistribution of molecules in cell membrane may result in budding and shedding of vesicles smaller than micrometer, called microvesicles. Microvesicles reflect in their cha\u00adracteristics the state and type of cells wherefrom they originate. The state of cells may be changed in diseases therefore also microvesicles, which originate from those cells, characte\u00adristically represent the disease [&hellip;]<\/p>\n","protected":false},"author":27,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[389,411,413],"tags":[],"class_list":["post-4125","post","type-post","status-publish","format-standard","hentry","category-2-2010-archive","category-biophysics","category-cell-biology"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Lateral redistribution of molecules in cell membrane may result in budding and shedding of vesicles smaller than micrometer, called microvesicles. Microvesicles reflect in their cha\u00adracteristics the state and type of cells wherefrom they originate. The state of cells may be changed in diseases therefore also microvesicles, which originate from those cells, characte\u00adristically represent the disease [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/\" \/>\n<meta property=\"og:site_name\" content=\"Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"article:published_time\" content=\"2013-09-08T12:06:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2013-09-09T10:23:05+00:00\" \/>\n<meta name=\"author\" content=\"Danaja \u017dolger\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Danaja \u017dolger\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/\"},\"author\":{\"name\":\"Danaja \u017dolger\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/73480dbc9a2cf58475cea889e2532c9d\"},\"headline\":\"Microvesicles from Blood Plasma Observed by Electron Microscopy\",\"datePublished\":\"2013-09-08T12:06:01+00:00\",\"dateModified\":\"2013-09-09T10:23:05+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/\"},\"wordCount\":144,\"commentCount\":0,\"articleSection\":[\"2\",\"Biophysics\",\"Cell Biology\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/\",\"name\":\"Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#website\"},\"datePublished\":\"2013-09-08T12:06:01+00:00\",\"dateModified\":\"2013-09-09T10:23:05+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/73480dbc9a2cf58475cea889e2532c9d\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/medrazgl.si\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Microvesicles from Blood Plasma Observed by Electron Microscopy\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#website\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/\",\"name\":\"Dru\u0161tvo Medicinski razgledi\",\"description\":\"Dru\u0161tvo Medicinski razgledi\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/medrazgl.si\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/73480dbc9a2cf58475cea889e2532c9d\",\"name\":\"Danaja \u017dolger\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g\",\"caption\":\"Danaja \u017dolger\"},\"url\":\"https:\\\/\\\/medrazgl.si\\\/en\\\/author\\\/danaja-zolger\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/","og_locale":"en_US","og_type":"article","og_title":"Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi","og_description":"Lateral redistribution of molecules in cell membrane may result in budding and shedding of vesicles smaller than micrometer, called microvesicles. Microvesicles reflect in their cha\u00adracteristics the state and type of cells wherefrom they originate. The state of cells may be changed in diseases therefore also microvesicles, which originate from those cells, characte\u00adristically represent the disease [&hellip;]","og_url":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/","og_site_name":"Dru\u0161tvo Medicinski razgledi","article_published_time":"2013-09-08T12:06:01+00:00","article_modified_time":"2013-09-09T10:23:05+00:00","author":"Danaja \u017dolger","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Danaja \u017dolger","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/#article","isPartOf":{"@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/"},"author":{"name":"Danaja \u017dolger","@id":"https:\/\/medrazgl.si\/#\/schema\/person\/73480dbc9a2cf58475cea889e2532c9d"},"headline":"Microvesicles from Blood Plasma Observed by Electron Microscopy","datePublished":"2013-09-08T12:06:01+00:00","dateModified":"2013-09-09T10:23:05+00:00","mainEntityOfPage":{"@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/"},"wordCount":144,"commentCount":0,"articleSection":["2","Biophysics","Cell Biology"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/","url":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/","name":"Microvesicles from Blood Plasma Observed by Electron Microscopy - Dru\u0161tvo Medicinski razgledi","isPartOf":{"@id":"https:\/\/medrazgl.si\/#website"},"datePublished":"2013-09-08T12:06:01+00:00","dateModified":"2013-09-09T10:23:05+00:00","author":{"@id":"https:\/\/medrazgl.si\/#\/schema\/person\/73480dbc9a2cf58475cea889e2532c9d"},"breadcrumb":{"@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/medrazgl.si\/mikrovezikli-iz-krvne-plazme-opazovani-z-elektronsko-mikroskopijo\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/medrazgl.si\/en\/"},{"@type":"ListItem","position":2,"name":"Microvesicles from Blood Plasma Observed by Electron Microscopy"}]},{"@type":"WebSite","@id":"https:\/\/medrazgl.si\/#website","url":"https:\/\/medrazgl.si\/","name":"Dru\u0161tvo Medicinski razgledi","description":"Dru\u0161tvo Medicinski razgledi","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/medrazgl.si\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/medrazgl.si\/#\/schema\/person\/73480dbc9a2cf58475cea889e2532c9d","name":"Danaja \u017dolger","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/4afad68de2e1f0fcbdffe28c1babd32d590ec613992499e4dcf0229abce21eba?s=96&d=mm&r=g","caption":"Danaja \u017dolger"},"url":"https:\/\/medrazgl.si\/en\/author\/danaja-zolger\/"}]}},"_links":{"self":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts\/4125","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/comments?post=4125"}],"version-history":[{"count":0,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts\/4125\/revisions"}],"wp:attachment":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/media?parent=4125"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/categories?post=4125"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/tags?post=4125"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}