{"id":4845,"date":"2013-09-13T12:42:39","date_gmt":"2013-09-13T12:42:39","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=4845"},"modified":"2013-09-13T12:43:38","modified_gmt":"2013-09-13T12:43:38","slug":"determination-of-estrogen-receptors-in-small-intestinal-mucosa","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/determination-of-estrogen-receptors-in-small-intestinal-mucosa\/","title":{"rendered":"Determination of estrogen receptors in small intestinal mucosa"},"content":{"rendered":"<p>Small intestinal mucosa of rats was tested for the presence of estrogen receptors. For determination of estrogen receptors\u00a0\u00a03H-estradiol 17-beta binding assay with or with our excess\u00a01H-estradiol 17-beta was used. Three sub cellular fractions including nuclei, microsomes and cytosol, were analyzed. Some animals were pretreated with\u00a01H-estradiol in order to determine whether it induces an increase in specific binding of estradiol. Estrogen receptors were never found, either in cytosol or in microsomes. The nuclear fraction obtained from the intestinal mucosa of non.treated animals, showed low specific binding (63fmol\/mg of protein), while in rats treated with estradiol this value increased by approx. nine times as compared to control values\u00a0(600fmol\/mg).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Small intestinal mucosa of rats was tested for the presence of estrogen receptors. For determination of estrogen receptors\u00a0\u00a03H-estradiol 17-beta binding assay with or with our excess\u00a01H-estradiol 17-beta was used. Three sub cellular fractions including nuclei, microsomes and cytosol, were analyzed. Some animals were pretreated with\u00a01H-estradiol in order to determine whether it induces an increase in [&hellip;]<\/p>\n","protected":false},"author":26,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[297,420,408],"tags":[],"class_list":["post-4845","post","type-post","status-publish","format-standard","hentry","category-3-1991-archive","category-physiology","category-preclinical-research-paper"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Determination of estrogen receptors in small intestinal mucosa - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/ugotavljanje-navzocnosti-estrogenskih-receptorjev-v-sluznici-tankega-crevesja\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Determination of estrogen receptors in small intestinal mucosa - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Small intestinal mucosa of rats was tested for the presence of estrogen receptors. For determination of estrogen receptors\u00a0\u00a03H-estradiol 17-beta binding assay with or with our excess\u00a01H-estradiol 17-beta was used. Three sub cellular fractions including nuclei, microsomes and cytosol, were analyzed. 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