{"id":6144,"date":"2013-09-26T15:29:46","date_gmt":"2013-09-26T15:29:46","guid":{"rendered":"http:\/\/test-medrazgl.aed-baza.si\/?p=6144"},"modified":"2013-09-26T15:29:46","modified_gmt":"2013-09-26T15:29:46","slug":"molecular-diagnosis-of-tuberculosis-hope-or-illusion","status":"publish","type":"post","link":"https:\/\/medrazgl.si\/en\/molecular-diagnosis-of-tuberculosis-hope-or-illusion\/","title":{"rendered":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion?"},"content":{"rendered":"<p>Tuberculosis represents a great world public health problem due to its increased incidence and the advent of multidrug-resistant strains. Recent developments in molecular microbiology have raised hopes about the possibilities of new strategies for tuberculosis diagnosis. Nucleic acid amplification of target molecules to a detectable level is the best developed and the best analyzed system for detecting mycobacteria. A variety of different amplification techniques has been described recently. Although these techniques promise to reduce the time for diagnosis from weeks to hours, their main drawback is lower sensitivity in comparison to the culture. Besides their potential value in diagnosis, amplification techniques offer the possibility of a rapid identification and drug susceptibility determination. Their application in other fields such as epidemiology, could benefit the control of tuberculosis indirectly. This article presents a brief overview of the principles of molecular diagnosis of tuberculosis, summarizes some of the application problems, and describes three recently developed commercial amplification tests based on polymerase chain reaction or transcription-mediated RNA amplification.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tuberculosis represents a great world public health problem due to its increased incidence and the advent of multidrug-resistant strains. Recent developments in molecular microbiology have raised hopes about the possibilities of new strategies for tuberculosis diagnosis. Nucleic acid amplification of target molecules to a detectable level is the best developed and the best analyzed system [&hellip;]<\/p>\n","protected":false},"author":21,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[323,432],"tags":[],"class_list":["post-6144","post","type-post","status-publish","format-standard","hentry","category-4-1996-archive","category-microbiology-and-immunology"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"og:description\" content=\"Tuberculosis represents a great world public health problem due to its increased incidence and the advent of multidrug-resistant strains. Recent developments in molecular microbiology have raised hopes about the possibilities of new strategies for tuberculosis diagnosis. Nucleic acid amplification of target molecules to a detectable level is the best developed and the best analyzed system [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/\" \/>\n<meta property=\"og:site_name\" content=\"Dru\u0161tvo Medicinski razgledi\" \/>\n<meta property=\"article:published_time\" content=\"2013-09-26T15:29:46+00:00\" \/>\n<meta name=\"author\" content=\"Ana Dov\u010d\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ana Dov\u010d\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/\"},\"author\":{\"name\":\"Ana Dov\u010d\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/d5357cbe600d0d917011600944e395d3\"},\"headline\":\"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion?\",\"datePublished\":\"2013-09-26T15:29:46+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/\"},\"wordCount\":171,\"commentCount\":0,\"articleSection\":[\"4\",\"Microbiology and Immunology\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/\",\"name\":\"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#website\"},\"datePublished\":\"2013-09-26T15:29:46+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/d5357cbe600d0d917011600944e395d3\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/medrazgl.si\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#website\",\"url\":\"https:\\\/\\\/medrazgl.si\\\/\",\"name\":\"Dru\u0161tvo Medicinski razgledi\",\"description\":\"Dru\u0161tvo Medicinski razgledi\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/medrazgl.si\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/medrazgl.si\\\/#\\\/schema\\\/person\\\/d5357cbe600d0d917011600944e395d3\",\"name\":\"Ana Dov\u010d\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g\",\"caption\":\"Ana Dov\u010d\"},\"url\":\"https:\\\/\\\/medrazgl.si\\\/en\\\/author\\\/ana-dovc\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/","og_locale":"en_US","og_type":"article","og_title":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi","og_description":"Tuberculosis represents a great world public health problem due to its increased incidence and the advent of multidrug-resistant strains. Recent developments in molecular microbiology have raised hopes about the possibilities of new strategies for tuberculosis diagnosis. Nucleic acid amplification of target molecules to a detectable level is the best developed and the best analyzed system [&hellip;]","og_url":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/","og_site_name":"Dru\u0161tvo Medicinski razgledi","article_published_time":"2013-09-26T15:29:46+00:00","author":"Ana Dov\u010d","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Ana Dov\u010d","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/#article","isPartOf":{"@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/"},"author":{"name":"Ana Dov\u010d","@id":"https:\/\/medrazgl.si\/#\/schema\/person\/d5357cbe600d0d917011600944e395d3"},"headline":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion?","datePublished":"2013-09-26T15:29:46+00:00","mainEntityOfPage":{"@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/"},"wordCount":171,"commentCount":0,"articleSection":["4","Microbiology and Immunology"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/","url":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/","name":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion? - Dru\u0161tvo Medicinski razgledi","isPartOf":{"@id":"https:\/\/medrazgl.si\/#website"},"datePublished":"2013-09-26T15:29:46+00:00","author":{"@id":"https:\/\/medrazgl.si\/#\/schema\/person\/d5357cbe600d0d917011600944e395d3"},"breadcrumb":{"@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/medrazgl.si\/molekularna-diagnostika-tuberkuloze-upanje-ali-iluzija\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/medrazgl.si\/en\/"},{"@type":"ListItem","position":2,"name":"Molecular Diagnosis of Tuberculosis \u2013 Hope or Illusion?"}]},{"@type":"WebSite","@id":"https:\/\/medrazgl.si\/#website","url":"https:\/\/medrazgl.si\/","name":"Dru\u0161tvo Medicinski razgledi","description":"Dru\u0161tvo Medicinski razgledi","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/medrazgl.si\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/medrazgl.si\/#\/schema\/person\/d5357cbe600d0d917011600944e395d3","name":"Ana Dov\u010d","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/c594b0ce318244f2cfcde220744f16d9c708376514d2de7d1e0e1f11e08fd347?s=96&d=mm&r=g","caption":"Ana Dov\u010d"},"url":"https:\/\/medrazgl.si\/en\/author\/ana-dovc\/"}]}},"_links":{"self":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts\/6144","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/users\/21"}],"replies":[{"embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/comments?post=6144"}],"version-history":[{"count":0,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/posts\/6144\/revisions"}],"wp:attachment":[{"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/media?parent=6144"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/categories?post=6144"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medrazgl.si\/en\/wp-json\/wp\/v2\/tags?post=6144"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}