Effect of Different Ethanol Concentrations on Phosphatidylethanol and Phosphatidic Acid Formation in the Human Neuroblastoma SH-SY5Y Cells
The effect of ethanol on the process of signal transduction was studied in the human neuroblastoma SH-SY5Y cells. By dissolving in the cell membrane ethanol changes its chemical and physical structure. In the presence of membrane enzyme phospholipase D, a specific product phosphatidylethanol is formed, at the expense of normal formation of phosphatidic acid. Higher ethanol concentrations (25mmol/l, 50mmol/l, 100mmol/l, 150mmol/l) increasingly inhibit phosphatidic acid formation and stimulate phosphatidylethanol synthesis. Phosphatidylethanol is formed exclusively at the expense of phosphatidic acid and no other mechanism of phosphatidic acid synthesis is present. The formation of phosphatidic acid is completely inhibited by adding 150mmol/l ethanol.