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Archive » 1996 » 1 » | Archive » Medical field » Fields » Microbiology and Immunology »

Quantitative Polymerase Chain Reaction – a Novel Method for Monitoring the Course and Treatment of Viral Diseases

 
Abstract:

This post is also available in: enEnglish slSlovenščina (Slovenian)

The polymerase chain reaction is a relatively new in vitro method, which uses enzymatic synthesis to amplify, in an exponential manner, specific DNA or RNA sequences. The polymerase chain reaction can be used both to detect the presence of microbial sequences and to provide a quantitative evaluation of the number of copies of the genome present. This article presents a brief overview of the principles of quantitative polymerase chain reaction, summarizes some of the application problems, and describes two recently developed simple and rapid assays for quantitative detection of hepatitis C virus and human immunodeficiency virus RNA in serum and plasma samples. Both assays were found to be valuable tools for determining the natural history of infection, dissecting viral pathogenesis and monitoring the efficacy of therapeutic interventions.

Authors:
Seme Katja, Poljak Mario

Keywords:
virus diseases, polymerase chain reaction

Cite as:
Med Razgl. 1996; 35: 59–74.

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