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Sex hormones, which are involved in the development and maintenance of reproductive tissue, are produced by female and male gonads, suprarenal cortex and the placenta. There are three main sex hormone groups: estrogens, gestagens and androgens. Natural, native-synthetic and synthetic hormones are used in hormonal therapy. The paper presents the characteristics of different hormonal substances. Knowledge of the different biological effects of sex hormones facilitates the clinical use of these hormones in hormonal therapy in the fields of gynecology and andrology.