Elevated plasma fibrinogen concentration is a risk factor for cardiovascular diseases. In addition to several factors such as advanced age, diabetes mellitus, cardiovascular diseases, elevated plasma cholesterol and glucose, and smoking, also genetic factors have been shown to influence plasma fibrinogen level. Studies on influence of TaqI, HaeIII and BclI polymorphisms on plasma fibrinogen concentration have shown contradictory results. Therefore, the influence of TaqI, HaeIII and BclI polymorphisms on plasma fibrinogen concentration was investigated in healthy subjects from Slovenia. 143 healthy volunteers were included in the cross-sectional study. Fibrinogen values were determined with a clotting assay. Polymorphisms were detected by polymerase chain reaction and digestion with Taql, HaeIII and BclI restriction enzymes. Statistical analysis was performed with Student’s t-test, analysis of variance, χ² test and multiple regression analysis. ln all individuals mean plasma fibrinogen concentration was 2.78 g/l (95% confidence interval 2.65–2.92 g/l), in smokers higher than in non-smokers (not significant; p = 0.40). Polymorphism TaqI was not associated with plasma fibrinogen level. There was a significant association between plasma fibrinogen concentration and HaeIII (p = 0.04) and BclI (p = 0.04) polymorphisms in the group of non-smokers. 5% of the plasma fibrinogen variance in non-smokers could be attributed to the HaeIII polymorphism (p = 0.03). ln smokers, there was no association between HaeIII and BclI polymorphisms and plasma fibrinogen level.