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The purpose of this study was to de­termine the diagnostic value of the lymphocyte blast transformation test in Lyme disease. Our laboratory results were compared with the diag­noses established by physicians. The course of the illness was followed in 213 individuals who­se blood samples were tested for cell immunity against Borrelia burgdorferi. All patients sho­wed clinical picture compatible with Borrelia burgdorferi infection. Sixty-five of the 213 patients (30.5%) developed cellular immunity, 4 (1.9%) showed a non-specific cellular immune respon­se, and 144 patients (67.6%) showed no cellu­lar immune response to Borrelia burgdorferi antigens. The individuals were further divided into 5 groups by their clinical signs. In group 1, cel­lular immune response was observed in 45.8 per cent of patients, in group 2 in 57.1 per cent, in group 3 in 11.1 per cent, in group 4 22.1 per cent and in group 5 37.2 per cent of patients.