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Doping involves the use of substances and methods prohibited by international and natio­nal sports institutions. The use of certain substances and methods is harmful to the human body, therefore all sports organizations are trying to protect athletes from the harmful side effects of such substances and methods. The article discusses individual classes of prohibi­ted substances, including stimulants, narcotics, anabolic agents, diuretics, peptide hormones and their analogues, blood doping, alcohol, marihuana, local anesthetics, corticosteroids and beta-blockers. A broader view on doping in sports is presented, as well as regulations on do­ping. The current list of prohibited substances and methods is added as well.