BACKGROUND. Infant tactile stimulation reduces distress behaviours, improves pulmonary function, weight gain per day, bone mineralization, developmentally appropriate functioning, enhances sleep organization and early attachment between infant and parents. We wanted to investigate the effect of tactile stimulation on physiological stability, behavioural response and phase adjustment of rest-activity rhythms to the nocturnal period in full-term infants. METHODS. Study population consisted of 22 newborns, whose postmenstrual age corresponded to 37-42 weeks, mean age at the day of inclusion was 20,09 (min = 9 days, max = 47 days, SD = 9,68). Children were given 15 minute tactile stimulations on three subsequent days. Stability of physiological parameters was recorded using cardiorespirography, blood pressure and body temperature were recorded before and after stimulation and infant’s behaviour was observed according to Naturalistic Observation of Newborn Behaviour Sheet. Rest-activity cycles were measured actigraphicaly from three days prior to, until three days after stimulation procedures. RESULTS. After stimulation maximal frequency of heartbeat on day one accelerated (Wilcoxon’s test, p = 0,016), and so did the mean frequency of heartbeat on day three (p = 0,012). Differences in hemoglobin oxigen saturation, blood pressure and body temperature were all statistically insignificant. Newborns exhibited more selfregulatory behaviors after stimulation (Wilcoxon’s test, first day p = 0,018, third day p = 0,016) and also more stress behaviors (third day: p = 0,030). Activity index was significantly higher for the daytime (p < 0,01) and lower for the night time period (p < 0,01) after short term tactile stimulation. CONCLUSIONS. Tactile stimulation is a safe method. Newborns showed more selfregulative than stress behaviour. After short term tactile stimulation infants showed a favourable adjustment of rest-activity cycles.