BACKGROUNDS. Numerous scientific and clinical findings confirm that pain slows down the process of wound healing. In our previously published research, we presented a prototype of an analgesic wound dressing with two analgesics: lidocaine, a local anesthetic with immediate onset of effect for alleviation of pain caused by wound care, and diclofenac for further reduction of the pain present due to the injury itself. The goal of the study reported in this paper was to evaluate the safety of this prototype. For this purpose, we tested biocompatibility of the materials used in the multilayered wound dressing with and without the inclusion of active substances. METHODS. In order to verify biocompatibility of the materials, we used human skin fibroblasts. The zone of inhibition was observed with two microscopes, cell vitality was tested with the Live/Dead kit. RESULTS. Regardless of the combination of materials — active substance, we did not discover any toxic effects on the cells. In the case of all three used materials; alginate, viscose, and polyethylene terephthalate (PET), we saw growth of fibroblasts in petri dishes, however the cells grew completely unaffected only with PET. In the case of PET with added lidocaine, there was even more cells than in the petri dish with PET only, reflecting the fact that there was no local overdose of lidocaine. CONCLUSIONS. Biocompatibility tests have confirmed the proper selection of layers in the prepared prototype dressing; PET, which will be due to its inertness the layer in contact with the wound, promotes the growth of skin fibroblasts. Despite the hydrophobic properties of the PET used, large pores, which are the result of rare weaving, freely leave the exudate into higher layers. The other two layers are also non-toxic, wherein viscose encourages the formation of the epithelial cell layer; the advantages of alginate were on the other side shown in numerous other studies.